Recombinant Human Apolipoprotein E, His (HEK293-expressed) 在 HEK293 細胞中表達,N 端帶有一個 His 標簽。Recombinant Human Apolipoprotein E (apoE) 以同工型依賴性方式促進膽固醇釋放。 Synonyms rHuApolipoprotein E, His; ApoE; Apolipoprotein E Species HumanSource HEK 293 Accession P02649 Gene ID 348 Molecular Weight Approximately 39.0 kDa AA Sequence KVEQAVETEP EPELRQQTEW QSGQRWELAL GRFWDYLRWV QTLSEQVQEE LLSSQVTQEL RALMDETMKE LKAYKSELEE QLTPVAEETR ARLSKELQAA QARLGADMED VCGRLVQYRG EVQAMLGQST EELRVRLASH LRKLRKRLLR DADDLQKRLA VYQAGAREGA ERGLSAIRER LGPLVEQGRV RAATVGSLAG QPLQERAQAW GERLRARMEE MGSRTRDRLD EVKEQVAEVR AKLEEQAQQI RLQAEAFQAR LKSWFEPLVE DMQRQWAGLV EKVQAAVGTS AAPVPSDNHH HHHHH Biological Activity Data is not available. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Apolipoprotein E, His (HEK293-expressed) (rHuApolipoprotein E, His) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability Lyophilized recombinant Human Apolipoprotein E, His (HEK293-expressed) (rHuApolipoprotein E, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background ApoE isoform specifically inhibits lipid-particle-mediated cholesterol release from neurons. Although apoE and a lipid particle are lipid acceptors, when apoE and a lipid particle form a complex, apoE on the particle surface inhibits the lipid particle-mediated cholesterol release from cells in an apoE-concentration-dependent manner[2]. |